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iscripttm cdna synthesis kit  (TaKaRa)
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TaKaRa iscripttm cdna synthesis kit
Iscripttm Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iscripttm reverse transcription supermix  (Bio-Rad)
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Bio-Rad iscripttm reverse transcription supermix
Iscripttm Reverse Transcription Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iscripttm select cdna synthesis kit  (Bio-Rad)
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Bio-Rad iscripttm select cdna synthesis kit
Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to <t>cDNA</t> for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.
Iscripttm Select Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iscripttm cdna synthese kit  (Bio-Rad)
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Bio-Rad iscripttm cdna synthese kit
Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to <t>cDNA</t> for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.
Iscripttm Cdna Synthese Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iscripttm advanced cdna conversion kit  (Bio-Rad)
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Bio-Rad iscripttm advanced cdna conversion kit
Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to <t>cDNA</t> for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.
Iscripttm Advanced Cdna Conversion Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iscripttm cdna synthesis kits  (Bio-Rad)
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Bio-Rad iscripttm cdna synthesis kits
Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to <t>cDNA</t> for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.
Iscripttm Cdna Synthesis Kits, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iscripttm gdna clr cdna kit  (Bio-Rad)
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Bio-Rad iscripttm gdna clr cdna kit
Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to <t>cDNA</t> for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.
Iscripttm Gdna Clr Cdna Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iscripttm gdna clear cdna synthesis kit  (Thermo Fisher)
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Thermo Fisher iscripttm gdna clear cdna synthesis kit
Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to <t>cDNA</t> for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.
Iscripttm Gdna Clear Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna synthesis kit iscripttm advanced  (Promega)
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Promega cdna synthesis kit iscripttm advanced
Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to <t>cDNA</t> for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.
Cdna Synthesis Kit Iscripttm Advanced, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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assays aurumtm total rna mini kit bio rad 7326820 iscripttm reverse transcription supermix  (Bio-Rad)
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Bio-Rad assays aurumtm total rna mini kit bio rad 7326820 iscripttm reverse transcription supermix
Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to <t>cDNA</t> for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.
Assays Aurumtm Total Rna Mini Kit Bio Rad 7326820 Iscripttm Reverse Transcription Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iscripttm reverse transcription supermix kit  (Bio-Rad)
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Bio-Rad iscripttm reverse transcription supermix kit
Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to <t>cDNA</t> for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.
Iscripttm Reverse Transcription Supermix Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to cDNA for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.

Journal: Cancer biology & therapy

Article Title: Garlic constituent diallyl trisulfide induced apoptosis in MCF7 human breast cancer cells.

doi: 10.4161/cbt.8.22.9882

Figure Lengend Snippet: Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to cDNA for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.

Article Snippet: 3 μg of the total extracted RNA was subjected to RT-PCR and cDNA was synthesized using the Biorad iScriptTM Select cDNA Synthesis kit (Biorad; Hercules, CA) containing random and oligo DT primer mix.

Techniques: Expressing, Control, Real-time Polymerase Chain Reaction, Staining, Immunofluorescence, Microscopy

Figure 7. DATS modulates survival and apoptotic signals in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM of DATS for 24 h. Nested RT-PCR and real-time RT-PCR of total RNA isolated from MCF-7 cells were performed. Non-treated cells were used as control. The products of PCR were electrophoresed on 1.5% Agarose gels (A). mRNA was extracted and reversed transcribed to cDNA for RT-PCR analysis of mRNA levels (B). Each data point is an average of three independent experiments and expressed as M ± SD and the significance was calculated.

Journal: Cancer biology & therapy

Article Title: Garlic constituent diallyl trisulfide induced apoptosis in MCF7 human breast cancer cells.

doi: 10.4161/cbt.8.22.9882

Figure Lengend Snippet: Figure 7. DATS modulates survival and apoptotic signals in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM of DATS for 24 h. Nested RT-PCR and real-time RT-PCR of total RNA isolated from MCF-7 cells were performed. Non-treated cells were used as control. The products of PCR were electrophoresed on 1.5% Agarose gels (A). mRNA was extracted and reversed transcribed to cDNA for RT-PCR analysis of mRNA levels (B). Each data point is an average of three independent experiments and expressed as M ± SD and the significance was calculated.

Article Snippet: 3 μg of the total extracted RNA was subjected to RT-PCR and cDNA was synthesized using the Biorad iScriptTM Select cDNA Synthesis kit (Biorad; Hercules, CA) containing random and oligo DT primer mix.

Techniques: Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Isolation, Control